Specific enzymatic amplification of DNA in vitro: The polymerase chain reaction.

Improvements that Mullis made to the polymerase chain reaction in 1983 enabled PCR to become a central technique in biochemistry and molecular biology. The process was first described by Kjell Kleppe and 1968 Nobel laureate H. Gobind Khorana. This was Mullis's first "methods" publication on the topic. With F. Faloona, S. Scharf, R. Saiki, G. Horn and H. Erlich.